TBO.com - TBO is Tampa Bay Online

Researchers Build DNA Strand

ADVERTISEMENT

By RICK WEISS, The Washington Post

Published: January 25, 2008

WASHINGTON - Scientists in Maryland on Thursday said they had built from scratch an entire microbial chromosome, a loop of synthetic DNA carrying all the instructions that a simple cell needs to live and reproduce.

The feat marks the first time that anyone has made such a large strand of hereditary material from off-the-shelf chemical ingredients. Previous efforts had yielded DNA strands less than one-twentieth the size, and those pieces lacked many of the key biological programs that tell a cell how to stay alive.

On the basis of earlier experiments, the researchers think the new, full-length loop would spontaneously "boot up" inside a cell, just as a downloaded operating system can awaken a computer - that would amount to the creation of the first truly artificial life form.

Team members emphasized that they have not done that yet but expressed confidence that they would do so before the end of the year.

"There are barriers ... but we are confident that they can be overcome," said J. Craig Venter, who led the effort with Daniel Gibson and Hamilton Smith at the J. Craig Venter Institute in Rockville. The work appeared in Thursday's online edition of the journal Science.

Venter said the goal is to design novel microbes whose handcrafted genomes endow them with the ability to produce useful chemicals, including renewable synthetic fuels that could substitute for oil.

Critics, however, countered that without better oversight of the fledgling field, synthetic biology is more likely to lead to the creation of potent biological weapons and runaway microbes that could wreak environmental havoc.

"Venter is claiming bragging rights to the world's longest length of synthetic DNA, but size isn't everything. The important question is not 'How long?' but 'How wise?'" said Jim Thomas of the ETC Group, a Montreal-based group that has called for a moratorium on the release and commercialization of synthetic organisms pending further public debate.

Venter's team started by determining the precise order of all 580,076 base pairs, or "letters" of DNA code, inside one of the simplest microbes known to science: Mycoplasma genitalium, a bacterium that can infect the human genital tract. The scientists bought small pieces of DNA, then perfected painstaking methods to stitch them together inside bacteria and yeast cells in exactly the right order.

The final product - 582,970 base pairs in all - is a near-exact replica of M. genitalium's genome, with a few intentional differences. The team omitted a DNA snippet that allows the microbe to infect other cells, for example, and added extra DNA as "watermarks" to differentiate their construct from the naturally occurring variety.

Share this:

Loading Comments...

Email Print

ADVERTISEMENT